BIOS 390 (BIOS 390)

BIOS 390 Week 6 LAB: Isolate Plasmid DNA-Once a plasmid has … introduced into competent bacterial cells and the cells have grown into colonies on a medium selective for cells containing the plasmid, the next step is to perform a miniprep of the plasmid DNA in preparation for DNA sequencing. The three steps are: 1) growing cells in liquid culture; 2) purifying the plasmid DNA from the culture; and 3) performing a restriction digest on the purified DNA to determine whether the DNA insert in the ...

Question Set 1 (TCO 3) DNA replication is said to be semiconservative because (TCO 3) DNA synthesis occurs in (TCO 3) Which of the following statements is correct? Question Set 2 (TCO 3) During DNA replication, DNA ligase is most active on the lagging strand. This is because (TCO 3) Phosphodiester bonds are formed between adjacent Okazaki fragments by (TCO 3) Which of the following activities are unique for DNA polymerase I? Question Set 3 (TCO 3) Once bound to an origin of replication, the o...

Gel electrophoresis is a laboratory technique used to separate DNA fragments and other macromolecules by size and charge. Gel electrophoresis is a technique in which fragments of DNA are … through a gel matrix by an electric current, and it separates DNA fragments according to size. A standard, or DNA ladder, is typically … so that the size of the fragments in the PCR sample can … determined. The results of a PCR reaction are usually … using gel electrophoresis……………………

BIOS 390 Week 4 LAB: Gene expression-Gene expression: it is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as transfer RNA or small nuclear RNA genes, the product is a functional RNA. The process of gene expression is used by all known life eukaryotes , prokaryotes , and utilized by viruses to generate the macromolecular machinery for life.Several steps in the gene express...

Question Set 1 (TCO 1) The basic principles of genetics contributed by Gregor Johann Mendel are: (TCO 1) The Augustinian monk, Gregor Johann Mendel, crossed yellow-seeded and green-seeded pea plants and then allowed the offspring to self-pollinate to produce an F2 The results were as follows: 6018 yellow and 2002 green (8020 total). The allele for green seeds has what relationship to the allele for yellow seeds? (TCO 1) In 1953, James Watson and Francis Crick proposed. Question Set 2 (TCO 1) I...

Polymerase chain … (PCR) is a laboratory technique to increase, or make many copies of, a specific target region of DNA in vitro. The goal of PCR is to make enough of the target DNA region that it can … analyzed or used in some other way. For example, DNA amplified by PCR may … sent for sequencing, visualized by gel electrophoresis, or cloned into a plasmid for further experiments…………………..document continueBIOS 390 Week 1 LAB 1

Question Set 1 (TCO 3) DNA replication is said to be semiconservative because (TCO 3) DNA synthesis occurs in (TCO 3) Which of the following statements is correct? Question Set 2 (TCO 3) During DNA replication, DNA ligase is most active on the lagging strand. This is because (TCO 3) Phosphodiester bonds are formed between adjacent Okazaki fragments by (TCO 3) Which of the following activities are unique for DNA polymerase I? Question Set 3 (TCO 3) Once bound to an origin of repli...

Question Set 1 (TCO 1) The basic principles of genetics contributed by Gregor Johann Mendel are: (TCO 1) The Augustinian monk, Gregor Johann Mendel, crossed yellow-seeded and green-seeded pea plants and then allowed the offspring to self-pollinate to produce an F2 The results were as follows: 6018 yellow and 2002 green (8020 total). The allele for green seeds has what relationship to the allele for yellow seeds? (TCO 1) In 1953, James Watson and Francis Crick proposed. Question Set 2 (T...

Polymerase chain … (PCR) is a laboratory technique to increase, or make many copies of, a specific target region of DNA in vitro. The goal of PCR is to make enough of the target DNA region that it can … analyzed or used in some other way. For example, DNA amplified by PCR may … sent for sequencing, visualized by gel electrophoresis, or cloned into a plasmid for further experiments…. document continues