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Molecular Diagnostics Bundle Set
Molecular Diagnostics Bundle Set
[Montrer plus]Molecular Diagnostics Bundle Set
[Montrer plus]Define Molecular Diagnostics - Answer Use of DNA, RNA and protein aka amino acid, to test for specific disease 
 
What is one of the way molecular diagnostic are use in the lab and gave an example? - Answer To speed up the identification of 
1) slow growing microrganism 
2) Fastidious microrgan...
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Ajouter au panierDefine Molecular Diagnostics - Answer Use of DNA, RNA and protein aka amino acid, to test for specific disease 
 
What is one of the way molecular diagnostic are use in the lab and gave an example? - Answer To speed up the identification of 
1) slow growing microrganism 
2) Fastidious microrgan...
3 common parts of a nucleotide - Answer 1) 5 carbon sugar 
2) phosphate group 
3) nitrogenous base 
 
Purines - Answer Adenine & Guanine 
 
Pyrimdines - Answer Cytosine, Uracil, and Thymine 
 
Nucleotide subunits are linked together by - Answer Phosphodiester bonds 
 
List three structural...
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Ajouter au panier3 common parts of a nucleotide - Answer 1) 5 carbon sugar 
2) phosphate group 
3) nitrogenous base 
 
Purines - Answer Adenine & Guanine 
 
Pyrimdines - Answer Cytosine, Uracil, and Thymine 
 
Nucleotide subunits are linked together by - Answer Phosphodiester bonds 
 
List three structural...
in one nucleotide of DNA what are the 3 major moieties (components) that make it up? - Answer - 5 carbon sugar 
- phosphate group 
- nitrogen bases 
 
what is the overall charge of DNA and on which moiety is it found - Answer DNA is overall negative, it is found on the oxygen of phosphate backbo...
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Ajouter au panierin one nucleotide of DNA what are the 3 major moieties (components) that make it up? - Answer - 5 carbon sugar 
- phosphate group 
- nitrogen bases 
 
what is the overall charge of DNA and on which moiety is it found - Answer DNA is overall negative, it is found on the oxygen of phosphate backbo...
What are the two major classes of nucleic acids? - Answer 1. deoxyribonucleic acid (DNA) 
2. Ribonucleic acid (RNA) 
 
What is structure of DNA? - Answer phosphodiester bonds connect deoxyribose sugars 
 
What are the bases in DNA? - Answer adenine 
guanine 
cytosine 
thymine 
 
What are the ...
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Ajouter au panierWhat are the two major classes of nucleic acids? - Answer 1. deoxyribonucleic acid (DNA) 
2. Ribonucleic acid (RNA) 
 
What is structure of DNA? - Answer phosphodiester bonds connect deoxyribose sugars 
 
What are the bases in DNA? - Answer adenine 
guanine 
cytosine 
thymine 
 
What are the ...
DNA contains deoxyribonucleotide triphosphates joined together by what type of covalent bond? - Answer Phosphodiester 
 
Nucleotides in DNA consist of what? - Answer Nitrogen Base, deoxyribose, and phosphate 
 
Which of the five ribose carbons of the deoxyribonucleotide are involved in formation...
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Ajouter au panierDNA contains deoxyribonucleotide triphosphates joined together by what type of covalent bond? - Answer Phosphodiester 
 
Nucleotides in DNA consist of what? - Answer Nitrogen Base, deoxyribose, and phosphate 
 
Which of the five ribose carbons of the deoxyribonucleotide are involved in formation...
trait - Answer particular characteristic 
 
Which elements make up the macromolecule of DNA? - Answer carbon, nitrogen, oxygen, phosphorous, and hydrogen 
 
What is the structure of DNA? - Answer phosphorylated ribose sugar (deoxy ribose) backbone with a nitrogenous base (guanine, adenine, cyt...
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Ajouter au paniertrait - Answer particular characteristic 
 
Which elements make up the macromolecule of DNA? - Answer carbon, nitrogen, oxygen, phosphorous, and hydrogen 
 
What is the structure of DNA? - Answer phosphorylated ribose sugar (deoxy ribose) backbone with a nitrogenous base (guanine, adenine, cyt...
A micoarray is testing for: - Answer a lot of genetic characteristics from one sample 
 
Southern Hybridization - Answer extract DNA 
probe w/ DNA 
 
Northern Hybridization - Answer extract RNA 
probe w/ DNA 
 
Western Hybridization - Answer extract proteins 
probe with antibodies 
 
What...
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Ajouter au panierA micoarray is testing for: - Answer a lot of genetic characteristics from one sample 
 
Southern Hybridization - Answer extract DNA 
probe w/ DNA 
 
Northern Hybridization - Answer extract RNA 
probe w/ DNA 
 
Western Hybridization - Answer extract proteins 
probe with antibodies 
 
What...
DNA contains deoxyribonucleotide triphosphates joined together by what type of covalent bond? 
A. aldehyde 
B. ester 
C. phosphodiester 
D. peptide - Answer phosphodiester 
 
What do phosphoiester bonds attach? - Answer hydroxyl oxygen of one phosphorylated ribose sugar to the phosphate phosphor...
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Ajouter au panierDNA contains deoxyribonucleotide triphosphates joined together by what type of covalent bond? 
A. aldehyde 
B. ester 
C. phosphodiester 
D. peptide - Answer phosphodiester 
 
What do phosphoiester bonds attach? - Answer hydroxyl oxygen of one phosphorylated ribose sugar to the phosphate phosphor...
Molecular Diagnostics - Answer Combination of laboratory medicine, molecular genetics and technology 
 
Molecular Diagnostics in Health Care - Answer Techniques allow detection of pathogenic mutations in nucleic acids to facilitate detection, diagnosis, classification, prognosis and therapy moni...
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Ajouter au panierMolecular Diagnostics - Answer Combination of laboratory medicine, molecular genetics and technology 
 
Molecular Diagnostics in Health Care - Answer Techniques allow detection of pathogenic mutations in nucleic acids to facilitate detection, diagnosis, classification, prognosis and therapy moni...
List the different specimens that may be submitted to a molecular diagnostics lab for testing - Answer Blood, Urine, stool, amniotic fluid, CSF, buccal cells. 
 
List 2 common anticoagulants used for molecular testing - Answer EDTA (for HIV, HCV, and CMV testing) and Acid Citrate Dextrose (for c...
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Ajouter au panierList the different specimens that may be submitted to a molecular diagnostics lab for testing - Answer Blood, Urine, stool, amniotic fluid, CSF, buccal cells. 
 
List 2 common anticoagulants used for molecular testing - Answer EDTA (for HIV, HCV, and CMV testing) and Acid Citrate Dextrose (for c...
Starting material, ending material, and active enzyme in process of replication is: - Answer DNA, DNA, DNA Polymerase 
 
Approximately how many genes are present in the human genome: - Answer 20,000-35,000 
 
What type of sugar molecule is present in DNA - Answer Deoxyribose 
 
What are the fo...
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Ajouter au panierStarting material, ending material, and active enzyme in process of replication is: - Answer DNA, DNA, DNA Polymerase 
 
Approximately how many genes are present in the human genome: - Answer 20,000-35,000 
 
What type of sugar molecule is present in DNA - Answer Deoxyribose 
 
What are the fo...
Which sample types must be amplified prior to analysis? - Answer buccal cells, saliva, bone, teeth, hair, dried blood, fixed tissue 
 
What are two ways to break down cell walls for DNA extraction? - Answer 1. homogenization through physical grinding 
2. treatment of detergent (SDS) with strong ...
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Ajouter au panierWhich sample types must be amplified prior to analysis? - Answer buccal cells, saliva, bone, teeth, hair, dried blood, fixed tissue 
 
What are two ways to break down cell walls for DNA extraction? - Answer 1. homogenization through physical grinding 
2. treatment of detergent (SDS) with strong ...
d (primers) - Answer *The specificity of the PCR reaction is determined by which of the following components?* 
a. Mg2+ concentration 
b. Deoxynucleotides 
c. DNA polymerase 
d. Primers 
 
a (primer dimers) - Answer *Complementarity of primer pairs at the 3' ends will result in what artifacts...
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Ajouter au panierd (primers) - Answer *The specificity of the PCR reaction is determined by which of the following components?* 
a. Mg2+ concentration 
b. Deoxynucleotides 
c. DNA polymerase 
d. Primers 
 
a (primer dimers) - Answer *Complementarity of primer pairs at the 3' ends will result in what artifacts...
The advantages of using real-time PCR to detect Chlyamidia and Gonorrhea is... - Answer - allow detection of organisms that are hard to culture 
- can be automated for high volume processing 
 
MALDI-TOF improves traditional culture methods by... - Answer -reduces need for biochem tests 
-pathog...
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Ajouter au panierThe advantages of using real-time PCR to detect Chlyamidia and Gonorrhea is... - Answer - allow detection of organisms that are hard to culture 
- can be automated for high volume processing 
 
MALDI-TOF improves traditional culture methods by... - Answer -reduces need for biochem tests 
-pathog...
The analysis of external specimens to assess technologist competency and assay performance (CAP survey) is which kind of testing? - Answer Proficiency 
 
A woman calls and asks that the results of her husband's HIV viral load assay be faxed to her. She gives the technologist her husband's name a...
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Ajouter au panierThe analysis of external specimens to assess technologist competency and assay performance (CAP survey) is which kind of testing? - Answer Proficiency 
 
A woman calls and asks that the results of her husband's HIV viral load assay be faxed to her. She gives the technologist her husband's name a...
What action should be taken if an unlabeled collection tube with a requisition for a factor V Leiden test is received in the laboratory. - Answer Notify the supervisor and reject the specimen. 
 
What action should be taken if after PCR, the amplification control has failed to yield a product? - A...
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Ajouter au panierWhat action should be taken if an unlabeled collection tube with a requisition for a factor V Leiden test is received in the laboratory. - Answer Notify the supervisor and reject the specimen. 
 
What action should be taken if after PCR, the amplification control has failed to yield a product? - A...
STR analysis is performed on bone marrow donor-recipient pairs prior to transplantation for which of the following reasons? - Answer to find informative alleles for post-transplantations studies 
 
Two elderly women who had been adopted into different families as infants discover circumstantially...
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Ajouter au panierSTR analysis is performed on bone marrow donor-recipient pairs prior to transplantation for which of the following reasons? - Answer to find informative alleles for post-transplantations studies 
 
Two elderly women who had been adopted into different families as infants discover circumstantially...
Which double-stranded DNA molecule has the highest melting temperature? 
A. An oligonucleotide with a repeating sequence of A-A-A at the 5 ́ end 
B. A molecule of 5,000 base pairs with a high number of A-T base pairs 
C. An oligonucleotide with a large number of repeating C-G-C codons 
D. A DNA pol...
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Ajouter au panierWhich double-stranded DNA molecule has the highest melting temperature? 
A. An oligonucleotide with a repeating sequence of A-A-A at the 5 ́ end 
B. A molecule of 5,000 base pairs with a high number of A-T base pairs 
C. An oligonucleotide with a large number of repeating C-G-C codons 
D. A DNA pol...
Phenotypic traits in Microbiology would be: - Answer Microscopic and colony morphology 
 
Enzyme or pigment production, carbohydrate fermentation patterns. - Answer Metabolic tests 
 
Prions are - Answer Proteins, which cause transmissable encephalopathies such as CreutzfeldtJakob disease 
 
O...
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Ajouter au panierPhenotypic traits in Microbiology would be: - Answer Microscopic and colony morphology 
 
Enzyme or pigment production, carbohydrate fermentation patterns. - Answer Metabolic tests 
 
Prions are - Answer Proteins, which cause transmissable encephalopathies such as CreutzfeldtJakob disease 
 
O...
The specificity of the PCR reaction is determined by which of the following components? - Answer Primers 
 
Complementarity of primer pairs at the 3′ ends will result in what artifacts? - Answer Primer dimers 
 
A DNA sequence with high GC content is more likely to have what property that inte...
Aperçu 3 sur 18 pages
Ajouter au panierThe specificity of the PCR reaction is determined by which of the following components? - Answer Primers 
 
Complementarity of primer pairs at the 3′ ends will result in what artifacts? - Answer Primer dimers 
 
A DNA sequence with high GC content is more likely to have what property that inte...
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