Fluorescence Study guides, Class notes & Summaries

When taking a fundus photo and you see a blue gray halo around the eye, you should move the camera correct answers closer As flurescein dye circulates throughout the eye and sequential pictures are taken,this is known as correct answers Angiograms For the slit lamp photography, in order for an image to be produced in each ocular and in the camera, a is necessary. correct answers Beam Splitter The reticule in the slit lamp and fundus camera is to compensate for the refractive error corre...

Light microscopy CORRECT ANSWER - Uses visible light to examine microscopic-sized objects that cannot be seen by the naked eye. - Allows finer detail than the naked eye. Electron microscopy CORRECT ANSWER - Visualize objects much smaller than those resolved optically. - Use accelerated electrons instead of light photons. - Samples are fixed with chemicals to cross-link molecules to preserve their structure. Fluorescent stains CORRECT ANSWER - Used with light microscopy. - Fluorescence ...

Resolution Correct Answer-small dist b/t 2 pts can be distinguished; D = .61λ/Nsinα, want lower D; Light microscope 200nM Bright field microscopy Correct Answer-normal light type Phase-contrast Correct Answer-altered phase interference of unobstructed light; looks like cells are surrounded by light DIC Correct Answer-3D looking cells Confocal Correct Answer-3D imaging with fluorescence, progessively capture full surface in focus by removing out of focus; normal point-scanning vs....

BIT 510 MidtermWhich of the following best describes the traditional central dogma of molecular biology? A. DNA is transcribed into mRNA which is translated into protein B. DNA can be produced by replication or reverse transcription C. DNA is replicated into RNA, some of which is translated into protein D. DNA is replicated into mRNA which is transcribed into protein - correct answer A. DNA is transcribed into mRNA which is translated into protein What is the end goal of cloning the Ca...

Light microscopy CORRECT ANSWER Limited ability to visualize bacterial components or distinguish between bacteria without doing something extra Electron microscopy CORRECT ANSWER Can view exterior and internal structures, smallest specimens Phase-contrast microscopy CORRECT ANSWER Refracts the light that goes through the specimen; can see detail, live cells Fluorescence microscopy CORRECT ANSWER Use of fluorochromes that emit light of specific wavelengths when excited by light of a diff...

CML vs. Leukemoid Reaction - ANSWER CML - decreased leukocyte alkaline phosphatase (LAP) Leukemoid Reaction - increased LAP Look-back - ANSWER The process for the identification and notification of recipients of blood components from prior donations of who now test positive for HCV, HIV-1 and/or HIV-2. External reference electrode used in ISE - ANSWER KCl The serum glucose concentration of a diabetic patient undergoing a 2-hr GTT should return to the baseline (fasting level) after ____...

Dr. Strummer prepares a Gram stain of a bacterial soil sample. Which of the following microscopy techniques is most appropriate for viewing his sample: A. Surface Electron Microscopy B. Brightfield Microscopy C. Fluorescence Electron Microscopy D. Transmission Electron Microscopy Correct Answer-B Microorganisms are... A. any organism too small to be seen w/o a microscope B. prokaryotes too small to be seen w/o a microscope C. eukaryotes too small to be seen w/o a microscope D. pro...

BIO 353 test 1 multiple choice -ASU questions & answers 2024 A student in a cellular biology laboratory is faced with the task of detecting protein X in muscle cells taken from a rat. The tissue is required to be live while under analysis. Which type of microscopy should be employed? a) Phase contrast light microscopy b) Transmission electron microscopy c) Scanning electron microscopy d) Fluorescence microscopy e) None of the above - ANSWER-d) Fluorescence microscopy Which...

beta-oxidation correct answer: enzymes repeatedly split off pairs of they hydrogenated carbon atoms that make up a fatty acid and join each pair to coenzyme A to form acetyl-CoA Chloroplasts correct answer: light harvesting structures found in photosynthetic eukaryotes; synthesize a few polypeptides compare and contrast archaeal flagella with bacterial flagella correct answer: archaeal are about half as thick as bacteria; not hollow; lack a central channel; flow together; powered by ATP...

Bright Field Microscope CORRECT ANSWER Most widely used Dark object, bright background Examine stained and unstained specimens Made by putting light through specimen Dark field microscope CORRECT ANSWER Image is lighter than illuminated background Uses a condenser on the light microscope Phase contrast microscope CORRECT ANSWER Used with live specimen Produces image with specimen against gray background Can see internal cells Scanning electron microscope CORRECT ANSWER Detailed 3D...