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Samenvatting cytologie cel II prof. Van Hengel $3.92   Add to cart

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Samenvatting cytologie cel II prof. Van Hengel

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Samenvatting van partim cytologie van cel II. Jaar (prof. Van Hengel). Bevat alles wat in de lessen is gezegd wat op de ppt (haar cursus) staat. 26 bladzijden, vaak verduidelijking met een figuur. Voor het examen leer ik alleen nog maar dit. Succes!

Last document update: 6 year ago

Preview 3 out of 26  pages

  • Unknown
  • December 31, 2017
  • January 14, 2018
  • 26
  • 2017/2018
  • Summary

2  reviews

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By: luciencabes14 • 4 months ago

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By: SamenvattingenGeneeskundeGent • 4 months ago

Translated by Google

Thank you so much! Good luck with cell 2!

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By: user10 • 2 year ago

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Available practice questions

Flashcards 11 Flashcards
Free 11 sales

Some examples from this set of practice questions

1.

Contrasteren= .... En geef 3 stoffen hiervoor

Answer: verhogen vd densiteitsverschillen dmv zware metalen, bij EM - uranylacetaat - loodcitraat - Os04 (osmiumtetroxide)

2.

Geef 4 soorten specifieke kleurtechnieken, wat kleuren ze en geef extra uitleg?

Answer: 1) Indifferente kleurstoffen --> vetten aankleuren bv. red oil 2) Immunohistochemische kleuring --> met antilichamen a) Immunocytochemische kleuring --> cel kleuren met Ig (bv. met DAB) b) Immuno- goud kleuring (EM) --> aan Ig hang je goudpartikel c) Immunofluorescentie (LM) --> aan Ig hang je een fluorochroom, direct/indirect en Toepassing:CTFR lokaliseren, is gemuteerd bij muco --> minder CFTR en niet meer apicaal bij muco patiënten.

3.

Pinholes, welke microscoop? resultaat

Answer: Confocle laser scanning microscoop (LM) --> scherper beeld

4.

3 lenssystemen LM + vergroting LM

Answer: oculair, objectief en condensor (hiermee richt je lichtbundel op weefsel) Max 1000x vergoot: oculair (10x) objectief (4x, 10x,40x,100x)

5.

Wat is er zo speciaal aan fluorescentie microscoop? Leg uit geef vb

Answer: Andere opstelling: emissie- en excitatiefilter Je grbuikt licht ve bepaalde golflengte= excitatielicht via filters , vervolgens wordt fluorochroom aangeslagen, licht komt uit materiaal= emissielicht bv. alexa 488 (abs 488,em519) Liefst aanslaan met 488 nm golflengte(excitatielicht) maar je kan niet één golflengte doorlaten --> enkel licht van 450- 550 nm doorlaten= excitatiefilter 2e filter= emissiefilter: laat enkel licht toe >515nm Alexa 488 w zichtbaar

6.

TEM vs SEM

Answer: transmissie vs scanning EM TEM: dun preparaat, binnenkant bekijken SEM: dikker preapraat, wordt in vacuüm afgescand en oppervlakte bekijken. Laagje goud/palladium--> geleidend vr elektricteit

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